Product Description
The Gene-Foci miniprep kit provides a fast, easy and cost-efficient way of lab scale (up to 20 μg) plasmid DNA preparation. This kit use silica matrix technology to bypass the labor intensive steps of phenol-chloroform extraction and ethanol DNA precipitation. The Gene-Foci miniprep kit utilizes enhanced SDS-alkaline method to lyse E.coli host cells. The silica matrix in the spin column selectively binds plasmid DNA in the cell lysate under high salt, low pH condition, then the washing steps eliminate contaminants，finally, the pure plasmid DNA is eluted from the silica matrix under low salt, high pH condition.
