Ni-NTA Agarose 6 FF
Ni-NTA Agarose 6 FF is ideal for purification of his-tagged proteins using immobilized metal ion affinity chromatography (IMAC). Proteins from any expression system can be purified under native or denaturing conditions. Binding of protein is based on the interaction between the polyhistidine tag of the recombinant protein and immobilized Ni2+ ions. Based on 6% crosslinked agarose, Ni-NTA Agarose 6 FF provides both higher binding capacity and higher flow rate than regular Ni-NTA resin based on 4% agarose.
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